The FLIPR assay was performed on FDSS/µCell with ligand addition at ten?s for your binding assay and mAb1/isotype addition at ten?s followed by hCCL1 addition at three hundred?s for the competition binding assay, with a total checking of 500?s. The excitation and emission wavelengths ended up set at 485?nm and 525?nm, respectively. Once the run, a